Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 β1-Integrin-Dependent Macrophage Migration is Regulated by MAP Kinase

 Takeichi SAKATA1, Seiji GODA2, Yumiko OGAWA3, Takashi IKEO2 and Shosuke MORITA3
1Graduate School of Dentistry (Oral and Maxillofacial Surgery),
2Department of Biochemistry,
2First Department of Oral and Maxillofacial Surgery,
Osaka Dental University, Osaka, Japan

J Oral Tissue Engin 2010;8(2): 124-132
SYNOPSIS
Chemokines regulate the homeostatic trafficking of lymphocytes and lymphocyte influx to sites of injury and inflammation. Here, we investigate the role of CXCL12 on the migration and signaling of RAW264 cells. We find that CXCL12-induced migration is enhanced in a dose-dependent manner, and CXCL12 enhances phosphorylation of ERK1/2 and AKT. Furthermore, this CXCL12-stimulated migration and phosphorylation is inhibited in the presence of the MEK1/2 inhibitor U0126, and the PI 3-K inhibitors, Wortmannin and LY294002. However, MEK1/2 inhibitor does not affect phosphorylation of the PI 3-K enzyme AKT, and PI 3-K inhibitors do not affect the phosphorylation of ERK1/2. This suggests that stimulation of ERK1/2 and AKT by CXCL12 occurs via an independent signaling pathway, which enhances migration of RAW264 cells.

Key words: β1-integrin, macrophage, MAP kinase