Journal of Oral Tissue Engineering

Influence of Root Dentin Surface Conditioning with Bone Morphogenetic Protein-2 on Periodontal Wound Healing in Beagle Dogs

Hirofumi MIYAJI1, Tsutomu SUGAYA1, Keisuke IBE2, Ryousuke ISHIZUKA1, Keisuke TOKUNAGA1 and Masamitsu KAWANAMI1
1Department of Periodontology and Endodontology, Division of Oral Health Science, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan
2Maruyama Kita 2jyo Dental Clinic, Sapporo, Japan

J Oral Tissue Engin 2011;8(3): 173-180

Bone morphogenetic protein-2 (BMP-2) may play a major role in osteoinduction and osteoclast differentiation. The influence of BMP-2 conditioning in graded doses to root dentin surface on periodontal wound healing was examined.
Twenty-four periodontal defects were surgically created on the buccal roots of four beagle dogs. The denuded root dentin surfaces were demineralized with 24% EDTA, 0.1 and 1.0 μg/μL BMP-2 solution (15 μL) was applied, and the groups were labeled as low-dose BMP (BL) and high-dose BMP (BH), respectively. In the control roots, phosphate-buffered saline was applied to the root surface. Specimens were histologically analyzed 16 weeks after surgery.
Periodontal wound healing was stimulated by conditioning with BMP-2. Formation of alveolar bone and cementum-like tissue was observed; however, in BH group, ankylosis and root resorption were accelerated in the defect area, and there was little evidence of periodontal ligament formation between alveolar bone and root surface.

Key words: bone morphogenetic protein-2, root dentin surface conditioning, periodontal wound healing, dog