Journal of Oral Tissue Engineering

An Attempt to Cell Differentiation in Three-dimensional Culture System Using Non-feeder ES-D3 Cells and Feeder Layer Type ES cells

Koichi IMAI1, Shoji TAKEDA1, Akito TANOUE2, Kazuaki NAKAMURA2, Kazuhiko SUESE3, Fumio WATARI4 and Hiromasa TAKASHIMA5
1Department of Biomaterials, Osaka Dental University, Osaka, Japan
2Department of Pharmacology, National Research Institute for Child Health and Development, Tokyo, Japan
3Osaka Dental University. School of Dental Technician and Hygienist, Osaka, Japan
4Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan
5Hatano Research. Institute., FDSC, Kanagawa, Japan

J Oral Tissue Engin 2011;8(3): 203-211

The embryonic stem cell test (EST) is an in vitro assay that has been developed to assess the embryotoxic potential of chemicals and biomaterials. An attempt for improvement in that ES cells used in the EST protocol are restricted to ES-D3 cells. If other kinds of ES cell become available, its experimental application will be further usefulness. We compared the incidence of pulsation between ES-D3 cells requiring no feeder cells for cultivation and EL M3 cells or ES-R1-EGFP B2/EGFP cells requiring feeder cells, to explore the experimental possibility of using ES cells requiring feeder cells.
As the present results with ES-D3 and EL M3 cells were similar to those obtained under the two-dimensional condition, these two kinds of cell are thought to be equally available under the present three-dimensional conditions. On the other hand, because ES-R1-EGFP B2/EGFP cells did not show any pulsation at all in the three-dimensional culture, other experimental conditions for the three-dimensional culture method need to be established with those cells. Besides, it was suggested that similar results could be obtained with EL M3 cells requiring feeder cells for cultivation compared to those with ES-D3 cells.

Key words: embryonic stem cell test, EST, feeder cells, EL M3 cells, ES-R1-EGFP B2/EGFP cells