Journal of Oral Tissue Engineering

Development of a New Barrier Membrane for Guided Bone Regeneration: an in Vitro and in Vivo Study

Kanako NORITAKE1, 2, Shinji KURODA1, Myat NYAN1, 2, Keiichi OHYA3, Yasuhiko TABATA4 and Shohei KASUGAI1, 2
1Oral Implantology and Regenerative Dental Medicine,
2Global Center of Excellence Program International Research Center for Molecular Science in Tooth and Bone Diseases,
3Pharmacology, Tokyo Medical and Dental University, Tokyo, Japan
4Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan

J Oral Tissue Engin 2011;9(2):53-63

Obtaining better outcomes from guided bone regeneration techniques often re-quires the use of ideal membranes. The objective of this study was to develop a novel membrane and investigate its contribution to the enhancement of bone regeneration.
The membrane was fabricated by combining β-TCP particles with dissolved gelatin hydrogel and cross-linking molecules with glutaraldehyde.
Rat bone-marrow cultured on this gelatin membrane for 14 days revealed time-dependent cell proliferation and the presence of alkaline-phosphatase-positive cells as well as that on a collagen membrane. Histological analysis indicated that significantly more new bone volume was generated in symmetrical full-thickness bone defects of rat calvariae covered by the gelatin membrane compared to that of the uncovered control defects at 4 and 8 weeks post operation.
The present study demonstrates that the novel β-TCP-containing gelatin hydrogel membrane stimulates bone regeneration. These results suggest the feasibility of this novel membrane for use in guided bone regeneration.

Key words: guided bone regeneration, membrane, bone marrow cells, bone defect