Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 Relationship between VEGF and AGEs on Periodontal Wound Healing in Model Rats with Type 2 Diabetes Mellitus

 Nobuhiro SHIGEMATSU1, Tomoo KONO2, Masatoshi UEDA2
1Graduate School of Dentistry (Periodontology),
2Department of Periodontology, Osaka Dental University, Osaka, Japan

J Oral Tissue Engin 2011;9(2):71-80
SYNOPSIS
VEGF is known to play a significant role in microangiopathy through AGEs. The objective of this study was to observe the expression and distribution of VEGF and AGEs immunohistochemically in the early healing stage of periodontal defects in model rats with type 2 diabetes (GK), and then clarify the relationship between these expressions and microangiopathy in the healing process.
We surgically prepared periodontal defects in the maxillary molars of male 30-week-old GK and SD rats. On Days 3, 5, and 7 after surgery, the rats were euthanized. The periodontal samples were stained immunohistochemically with anti-VEGF and anti-AGE monoclonal antibodies.
AGEs and VEGF were more significantly observed in the GK rats than in the SD rats around blood vessels. These results suggest that, in the GK rats under-going periodontal surgery, AGEs localized around capillary blood vessels contribute to an intensive expression of VEGF compared to the SD rats, and then VEGF causes microangiopathy during wound healing.

Key words: VEGF, AGEs, Periodontal wound healing, Type 2 diabetes mellitus