Journal of Oral Tissue Engineering

ORIGINAL ARTICLE
 Development of in Vitro Embryotoxicity Testing by Differentiation of Mouse ES cells using Glass Fiber

 Koichi IMAI1, Kazuhiko SUESE2, Akito TANOUE3, Kazuaki NAKAMURA3 and Hiromasa TAKASHIMA4
 1Department of Biomaterials, Osaka Dental University, Osaka, Japan
2Osaka Dental University. School of Dental Technician and Hygienist, Osaka, Japan
3Department of Pharmacology, National Research Institute for Child Health and Development, Tokyo, Japan
4Hatano Research. Institute, FDSC, Kanagawa, Japan

J Oral Tissue Engin 2011;9(2): 81-87
SYNOPSIS
We have proposed the embryotoxicity testing of mouse ES cells embedded in a collagen gel matrix. The rate of differentiation of ES-D3 cells into cardiomyocytes was measured using a glass fiber as a scaffold material for collagen gel. In order to design a test method for embryotoxicity under an exposure condition where broader applications would be possible in various fields using a glass fiber as a non-animal-derived scaffold material. We measured as the test materials using TEGDMA, EDGMA and 2HEMA. There was no significant difference found with glass fiber and collagen gel matrix. Therefore, it was suggested that glass fiber could be used as the scaffold material for in vitro embryotoxicity test. Furthermore; the Embryonic Stem Cell Test may be able to apply to the environmental field etc.

Key words: differentiation, ES-D3 cells, glass fiber, dental monomer