Journal of Oral Tissue Engineering

Effect of Hydroxyapatite Fiber Material with Autogenous Bone Graft on Vertical Bone Augmentation

Junichi KIMURA1, Makoto SHIOTA1, Kazuhiro KON1, Masaki FUJI1, Hitoshi SATO2 and Shouhei KASUGAI1
1Department of Oral Implantology and Regenerative Dental Medicine,
Tokyo Medical and Dental University, Tokyo, Japan
2Yaesu Chuo Dental Clinic, Tokyo Japan

J Oral Tissue Engin 2011;9(3):136-146

Purpose: The aim of this study was to examine effects of hydroxyapatite fiber (HF) combining with autogenous Bone on vertical bone augmentation.
Materials and methods: Polytetrafluoroethylene chambers were fixed to the parietal bone on the right and left sides in thirty-six Japanese male white rabbits. HF only (30 or 60 mg) and combination of HF with autogenous bone from tibia at different ratios (30 mg bone/HF ratio: 3/1, 3/2, 1/1) were grafted into each chamber. Chambers were left empty in the negative control animals (empty group). Rabbits were sacrificed at 4 and 8 weeks. Then, total bone volume (TBV) of highly calcified bone region in the chamber was quantified with micro-CT analysis. Histomor-phometric analysis was performed to evaluate the area of newly formed bone (BA). Statistical analysis was performed with the Student t-test and Tukey-Kramer's method.
Results: In histology, newly formed bone conducted by HF showed a particular structure, and active bone regeneration was observed in the groups containing more HF at 8 weeks. From 4 weeks to 8 weeks BA was significantly reduced in the control, HF 30 mg and ratio 3/1 groups. At 8 weeks BA of HF 60mg was signifi-cantly higher than the one of HF30mg group.
Conclusions: Combination of HF with autogenous bone was effective in vertical bone augmentation although optimal ratio of the combination needs further study.

Key words: hydroxyapatite fiber, bone augmentation, bone graft, bone substitute, osteoclast